An Enzymatic Spectrophotometric Method for the Determination of Homogentisic Acid in Plasma and Urine
نویسندگان
چکیده
منابع مشابه
An enzymatic spectrophotometric method for the determination of homogentisic acid in plasma and urine.
Previous methods for the determination of homogentisic acid have been based upon its ability to reduce silver (I), phosphomolybdic acid (a), or iodine (3-5). The error introduced by other biological substances which have similar reducing properties is inconsequential in the determination of the relatively large amounts of homogentisic acid present in urine from a patient with alcaptonuria. Such...
متن کاملDetermination of homogentisic acid in urine.
A relatively simple method for the quantitative determination of homogentisic acid in urine is presented. Homogentisic acid (2,5-dihydroxyphenylacetic acid) is oxidized by atmospheric oxygen in mild alkali to form 1,4-benzoquinone-2-acetic acid. The latter compound is then conjugated with 2,4-dinitrophenylhydrazine. The resulting hydrazone, in the presence of alcoholic sodium hydroxide, produce...
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In this simple, sensitive, and rapid enzymatic method for the determination of oxalate in urine or plasma, oxalate oxidase (EC 1.2.3.4) prepared from barley seedlings is used to convert oxalate to carbon dioxide and hydrogen peroxide, which is determined photometrically at 600 nm, with use of horseradish peroxidase, by oxidative coupling of 3-methyl-2-benzothiazoline hydrazine with N,N-dimethyl...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64307-8